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Category Archives for "Analysis"

Chemical analysis is a branch of chemistry which help to evaluate chemical compound. Tests for qulitative and quantitative analysis are discussed

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HPLC Chromatography | Its Principle and Working Methodology

HPLC analysis is one of the types of chromatography used to isolate and analyze mixtures. HPLC in full form is “High-pressure liquid chromatography.” Unlike column chromatography, here high pressure is employed in the process. Hence the name. But also due to its efficiency in the analysis of compounds, it is regarded as High-performance liquid chromatography. […]

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Ion Exchange chromatography | Principle, Method & Applications

Ion exchange chromatography is an interesting type of column chromatography. As you know, the Chromatography is a process of the separation of molecules from a mixture. This separation is done based on the differences in the adsorption coefficient or partition coefficient of the sample with the stationary phase. Whereas in ion exchange chromatography segregation of […]

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HPTLC-High performance thin layer chromatography

High performance thin layer chromatography

HPTLC sophisticated form of Thin-Layer chromatography (TLC). In full form, it is High-Performance Thin Layer Chromatography. This is so called because of the features like the greater efficiency and higher resolution. It has added advantages like auto-sampling, faster development of spots and also easy detection and quantification of separated compounds. The advancement concerning better efficiency […]

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Column Chromatography | Principle, Procedure & Applications

column chromatography

Column chromatography is the prototype of chromatography. It has simple instrumentation with minimal requirements. It works based on the principle of adsorption chromatography technique. Column Chromatography Principle Principle of involved in this technique is the separation of components by adsorption. The sample mixture is allowed to pass through a column of solid stationary phase under […]

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6 Difference between Chromatography and Electrophoresis

1 Difference between Chromatography and Electrophoresis

Chromatography is a technique which is meant for separation of component of a mixture. Similarly electrophoresis is also meant for separation of components of a given sample. But one needs to understand the¬†difference between chromatography and electrophoresis for analytical needs. In general electrophoresis is used mostly in biological labs and forensic analysis. But chromatography is […]

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Visible spectroscopy | Principle, Instrumentation & Applications

Visible spectroscopy is also called as colorimetry. This means it measures colored light (color+metry= color+ measurement). The Principle of Visible spectroscopy This is a study of the absorption of visible radiation within the wavelength range of 400nm to 800nm by any colored substance. Every colored substance absorbs light radiation of different wavelengths. When we plot […]

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