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Category Archives for "Analysis"

Chemical analysis is a branch of chemistry which help to evaluate chemical compound. Tests for qulitative and quantitative analysis are discussed

HPLC method: Procedure of HPLC test in brief

hplc method

HPLC method of chromatography is simple if one follows a well established procedure . Always keep the HPLC instrument clean including the chamber where the HPLC system is housed. Keep the room temperature to lower side and also fixed. Alteration in the room temperature during the HPLC method has effect on the detectors. All the solution, reagents, samples, […]

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Importance of Protein Analysis & the Assay Methods

Importance of Protein Analysis in diagnosis and research

Protein analysis is very important for Quantification of tissue or cell material in biological research. Understand body physiology For diagnosis of diseases by western blot, ELISA. To determine the mechanism of disease Quality control of packed food material and protein supplements. To develop vaccines. Biosynthesis of Hormones and Enzymes Forensic Investigation Identification of bacteria. Protein […]

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HPLCPumps: 3 Types and their Function

Hplc Pumps

HPLC pumps from the basic part of HPLC instrumentation. Unlike other chromatography techniques, HPLC needs to generate pressure by pumps. Without a proper pump, HPLC analysis is impossible and the instrument is not operable. As the name, HPLC i.e. high-performance liquid chromatography indicates, one needs to generate pressure. HPLC pumps generate pressure on the solvent so […]

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Gas Chromatography Theory of Operation & Instrumentation

Gas Chromatography Theory

Gas Chromatography Theory:  Gas chromatography relies on the separation of components of a volatile compound. Under the influence of a gaseous solvent flow which acts as mobile phase, the compounds are separated over a liquid stationary phase. The compounds having partition into gas elute first out of the column. While those with greater partition into stationary liquid phase elute out last. The carrier […]

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Gas Chromatography Principle, Instrumentation and Method

Gas Chromatography Principle

Gas chromatography principle is based on “partition” based separation of componenets. This chromatography is of two types like gas liquid chromatography (GLC) and gas solid chromatography (GSC). Of them GLC is widely used and so our entire discussion would be related to it. Gas Chromatography & principle (principle of GC): The principle in gas chromatography involves separation […]

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HPLC Parts; Accessories & their Function

hplc parts

High-performance liquid chromatography is shortly abbreviated as HPLC. This has a sophisticated and complex instrument set up to analyze components. The HPLC parts include an injector, a pump, column and detector. Other accessories include a computer, injector, solvent input and exhaust. Any problems in these parts can ultimately hinder the HPLC process. So it is important to procure […]

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HPLC injection: The technique and instrumentation

HPLC injection

HPLC injection is a technique used to load the sample into the HPLC system. This process is done using a specially designed injection system which is a separate and prominent part of the HPLC system. The most popular injector is the rheodyne injector. The injection is done manually by an external syringe or autoinjector. Then […]

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Hplc Mobile Phase: Requirement and precautions.

In High-performance liquid chromatography (HPLC) method, the mobile phase is of prime importance. It is the component which takes the actual process of separation under the influence of pressure over the stationary phase. The HPLC mobile phase is a bit of concern in comparison to mobile phase of other chromatography techniques due to the following reasons. ♠ […]

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HPLC Applications | In Diagnosis, Research & Industry

HPLC Applications

HPLC (high-performance liquid chromatography) is one of the advanced types of chromatography. It is highly sophisticated and expensive tools in the present analytical chemistry. It is given prominent importance due to its attributes like High sensitivity, i.e., the ability to evaluate samples of very minute concentrations like in nano-gram and picogram Detect precisely chemically similar molecules like […]

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