PCR Protocol | A Step by Step Procedure
The polymerase chain reaction, or PCR, was originally developed by Kary Mullis, for which he was awarded the Nobel Prize in 1993. PCR is a laboratory technique that is used to generate …
Analysis
Chemical analysis is a branch of chemistry which help to evaluate chemical compound. Tests for qulitative and quantitative analysis are discussed
Limit Test for Arsenic with Apparatus and Reaction
Arsenic is a heavy metal that is very harmful. Besides, as an impurity, it also creates instability in pharmaceutical preparations. However, it is impossible to avoid arsenic impurity from being present in …
Mass Spectrometry Principle, Types, Instrumentation & Applications
Mass spectrometry is used to determine the mass and molecular structure of a compound. Unlike other spectroscopy techniques, here the electromagnetic radiation is not used for the excitation of electrons. Further, there …
NMR Spectroscopy Principle, Instrumentation and Applications
Nuclear magnetic resonance spectroscopy is one that studies the spin changes at the nuclear level. This spin change occurs when a radio frequency energy is absorbed by the nucleus in the presence …
Limit Test for Iron with Principle and Procedure
Limit test for iron is a semi-quantitative test used to check the iron impurities in an official sample. Principle: This test is based on the reaction of thioglycolic acid with iron in …
Applications of Gas Chromatography: 5 Major Uses
Gas chromatography is one of the most widely used analytical tools. The applications of gas chromatography include Qualitative analysis Testing the purity of compounds To trace impurities Quantitative analysis This is the …
Fluorimetry | Principle, Instrumentation and Applications
Fluorimetry is a type of spectroscopy that measures the emitted radiation from a substance. This radiation is one that is emitted by the substance when the electrons transit from the excited state …
Potentiometric titration | Experimental Method and Applications
Potentiometric titration is a method of chemical analysis. This technique relies on the measurement of electromotive force (EMF) of a solution using a set of indicators and reference electrodes. The potential or …
Types of HPLC | Their Principle and Application
High performance liquid chromatography finds widespread application in analytical chemistry and biology. There are different types of HPLC like 1. Based on modes of chromatography : a) Normal phase mode b) Reverse …
Test for proteins | Chemical Methods of Identification in Labs
Proteins are the essential biomolecules present in the living organisms. They give structural support and rigidity to the organisms. There are many proteins like albumin, globulin, casein, gelatin, peptone etc. The tests …