List of buffer solutions | (Preparation Method for specific pH)

The list of buffer solutions include standard buffers and also specific buffers

Standard buffers are

  1. Hydrochloric acid buffer
  2. Acid phthalate buffer
  3. Phthalate buffer
  4. Phosphate buffer
  5. Alkaline borate buffer.

Specific buffers include

  1. Acetate bufferlist of buffer solutions
  2. Acetic ammonia buffer
  3. Acetone buffer
  4. Ammonia buffer
  5. Barbitone buffer
  6. Buffered Copper sulfate solution
  7. Glycerin solution
  8. Glycine buffer solution
  9. Imidazole buffer
  10. Palladium chloride buffered solution
  11. Phosphate buffer (mixed)

Standard buffers are the solutions which have a standard pH. They are used as reference standards for the measurement of pH. They are also used for chemical tests that need maintenance of specific pH.

The above mentioned standard buffers can be made into varying degrees of pH with appropriate combinations of solutions.

As you know, a buffer is a combination of strong acid and its basic salt or a strong base and its acidic salt.

Hydrochloric acid buffer is prepared by a combination of the hydrochloric acid solution with a potassium chloride solution.

Hydrochloric acid (0.2M): Hydrochloric acid is diluted with freshly boiled and cooled water to get 1000ml 7.292 g of HCl.

Potassium chloride (0.2M): 14.911 g of potassium chloride is dissolved in water and made to 1000ml.

pH Volume of 0.2M HCl in ml required
1.285.0
1.367.2
1.453.2
1.541.4
1.632.4
1.726.0
1.820.4
1.916.2
2.013.0
2.110.2
2.27.8

Acid Phthalate buffer

Take 50ml of 0.2M potassium hydrogen phthalate solution into a 200 ml volumetric flask. Then add below the mentioned volume of hydrochloric acids and make up the final volume with water.

pH Vol of 0.2M HCl (mL) to be used
2.2 49.5
2.442.2
2.635.4
2.828.9
3.022.3
3.215.7
3.410.4
3.66.3
3.82.9
4.00.1

Neutralized phthalate buffer: Into 200ml volumetric flask, add 50ml of 0.2M Potassium hydrogen phthalate solution. Then add the mentioned volume of 0.2M sodium hydroxide solution and make up the final volume with water.

pHVolume if 0.2M NaOH (ml)
4.2 3.0
4.4 6.6
4.6 11.1
4.8 16.5
5.0 22.6
5.2 28.8
5.4 34.1
5.6 38.8
5.8 42.3

Phosphate buffer:

Take 50ml of 0.2M of potassium dihydrogen phosphate solution into a 200ml flask. Then add 0.2m NaOH solution of defined volume in the table. Make up the final volume with water and shake the flask well.

pHVolume of 0.2M NaOH (mL)
5.83.6
6.05.6
6.28.1
6.411.6
6.616.4
6.822.4
7.029.1
7.234.7
7.439.1
7.642.4
7.844.5
8.046.1

Alkaline borate buffer:

Add 50ml of 0.2M boric acid and 0.2M potassium chloride in a 200ml volumetric flask. To this mixture add a specified volume of 0.2 M NaOH and makeup to the final volume.

pHVolume of 0.2N NaOH (mL)
8.0 3.9
8.2 6.0
8.4 8.6
8.611.8
8.815.8
9.020.8
9.226.4
9.432.1
9.636.9
9.840.6
1043.7

Leave a Comment