Category Archives for "Chemistry"

Chemistry is the science of study of matter in finest sense, its interactions and existence.

Limit Test for Iron with Principle and Procedure

Limit test for iron

Limit test for iron is a semi-quantitative test used to check the iron impurities in an official sample. Principle: This test is based on the reaction of thioglycolic acid with iron in the given sample. A purple colored ferrous thioglycolate salt is formed. The color intensity is compared with that of a standard substance containing […]

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Uses of Glass and its Applications to Man

uses of glass for storing pickles

The glass is one of the early discoveries of man. It was made out of molten sand. The uses of glass are indispensable, i.e., there will be no alternatives to it in some cases. Its applications to man include Kitchenware Eyeglasses and lenses Electronic Screens tv Microscopes for magnification Lights and bulbs Storage of specimens, […]

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Applications of Gas Chromatography | 5 Major Uses

applications of gas chromatography

Gas chromatography is one of the widely used analytical tools. The applications of gas chromatography include Qualitative analysis Testing the purity of compounds To trace impurities Quantitative analysis Determination of a mixture of drugs Isolation and identification of metabolites Identification of compound mixtures. Qualitative analysis: Here the compounds in a given sample are identified. Qualitative […]

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Fluorimetry | Principle, Instrumentation and Applications

Fluorescence spectroscopy

Fluorimetry is a type of spectroscopy which measures the emitted radiation from a substance. This radiation is one which is emitted by the substance when the electrons transit from excited state to ground state. The principle of fluorescence spectroscopy: In UV-Visible spectroscopy, the excitation wavelength is measured. But, here the emission wavelength is measured. When […]

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Azeotropic Distillation | With Two Examples and Applications

Azeotropic Distillation

You might have gone through different types of distillation techniques. Of them azeotropic distillation is quite interesting one. This method is used to separate azeotropic solutions. As per Wikipedia, an azeotrope is a mixture which cannot be separated by simple distillation. In general, most liquid mixtures can be separated by fractional distillation (FD). During the […]

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Types of HPLC | Their Principle and Application

types of hplc

High performance liquid chromatography finds widespread application in analytical chemistry and biology. There are different types of HPLC like 1. Based on modes of chromatography : a) Normal phase mode b) Reverse phase mode 2. Based on principle of separation :       c) Adsorption chromatography d) Ion exchange chromatography e) Ion pair chromatography f) Size exclusion […]

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