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Category Archives for "Chemistry"Chemistry is the science of study of matter in finest sense, its interactions and existence.
Mass Spectrometry Principle,Types, Instrumentation & Applications
Mass Spectrometry is an analytical technique used to determine the mass and molecular structure of a compound. Unlike other spectroscopy techniques, here the electromagnetic radiation is not used for excitation of electrons. Further, there is also no phenomenon of electrons moving in between the ground state and excited state. The principle of mass spectrometry is […]
Continue readingNMR Spectroscopy Principle, Instrument and Applications
Nuclear magnetic resonance spectroscopy is one that studies the spin changes at the nuclear level. This spin change occurs when a radio frequency energy is absorbed by the nucleus in the presence of a magnetic field. Principle of NMR spectroscopy In an atom with an odd mass number, the proton (nucleus) spins on its own […]
Continue readingLimit Test for Iron with Principle and Procedure
Limit test for iron is a semi-quantitative test used to check the iron impurities in an official sample. Principle: This test is based on the reaction of thioglycolic acid with iron in the given sample. A purple colored ferrous thioglycolate salt is formed. The color intensity is compared with that of a standard substance containing […]
Continue readingUses of Glass and its Applications to Man
The glass is one of the early discoveries of man. It was made out of molten sand. The uses of glass are indispensable, i.e., there will be no alternatives to it in some cases. Its applications to man include Kitchenware Eyeglasses and lenses Electronic Screens tv Microscopes for magnification Lights and bulbs Storage of specimens, […]
Continue readingApplications of Gas Chromatography | 5 Major Uses
Gas chromatography is one of the widely used analytical tools. The applications of gas chromatography include Qualitative analysis Testing the purity of compounds To trace impurities Quantitative analysis Determination of a mixture of drugs Isolation and identification of metabolites Identification of compound mixtures. Qualitative analysis: Here the compounds in a given sample are identified. Qualitative […]
Continue readingFluorimetry | Principle, Instrumentation and Applications
Fluorimetry is a type of spectroscopy which measures the emitted radiation from a substance. This radiation is one which is emitted by the substance when the electrons transit from excited state to ground state. The principle of fluorescence spectroscopy: In UV-Visible spectroscopy, the excitation wavelength is measured. But, here the emission wavelength is measured. When […]
Continue readingAzeotropic Distillation | With Two Examples and Applications
You might have gone through different types of distillation techniques. Of them azeotropic distillation is quite interesting one. This method is used to separate azeotropic solutions. As per Wikipedia, an azeotrope is a mixture which cannot be separated by simple distillation. In general, most liquid mixtures can be separated by fractional distillation (FD). During the […]
Continue readingPotentiometric titration | Experimental Method and Applications
Potentiometric titration is a method of chemical analysis. This technqiue relies on the measurement of electromotive force (EMF) of a solution using a set of indicator and reference electrodes. The potential or EMF of a solution depends on the nature and concentration of the ions of the test substance. The EMF or potential is measured […]
Continue readingTypes of HPLC | Their Principle and Application
High performance liquid chromatography finds widespread application in analytical chemistry and biology. There are different types of HPLC like 1. Based on modes of chromatography : a) Normal phase mode b) Reverse phase mode 2. Based on principle of separation : c) Adsorption chromatography d) Ion exchange chromatography e) Ion pair chromatography f) Size exclusion […]
Continue readingAnalytical Chemistry Examples | Their Uses and Applications
Analytical chemistry is a branch of chemistry which tries to estimate and determine the actual chemical entity in a given sample. When a packed food item is taken, we can find the details of its components to like carbohydrates, proteins, saturated fats, sodium chloride, etc. mentioned in grams or milligrams. This is ascertained by the […]
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