25 Differences between gram positive and gram negative bacteria

Bacteria are broadly classified as gram-positive and gram-negative bacteria based on gram stain. But both of the groups are Prokaryotic cells similar in many aspects. Check the similarities at the end after the table.

Differences between gram positive and gram negative bacteria

Differences between gram positive and gram negative bacteria

Sl.NoFeaturesGram +ve BacteriaGram -v Bacteria
1Gram Stain ReactionIn gram staining, the crystal the violet dye will be retained on
the cell wall after washing with the de-staining solution.
The de-staining solution will
remove the color of the crystal violet dye.
2Microscopic viewThe gram-positive bacteria will stain blue or purple color
under a microscope, because it retains the crystal violet dye
The gram-negative bacteria will stain pink or red color under a microscope because it accepts
the safranin dye.
3Cell wallIn the gram-positive bacteria the cell wall is a single layer
(contains plasma membrane)
In the gram-negative bacteria the cell wall is double layered (contains plasma membrane and outer membrane)
3Cell wall ShapeThe gram-positive bacteria contain flat and even cell walls.The gram-negative bacteria
contain wavy and uneven cell walls.
4ElasticityThe cell wall is very rigid, so it has less elasticity.The cell wall is less rigid, so it has high elasticity.
5PeptidoglycanThe proportion of
peptidoglycan present in the gram-positive bacteria is 80%
because the cell wall is very rigid.
The proportion of the
peptidoglycan present in the gram-negative bacteria is 2-12%, because of the cell wall has high elasticity.
6ThicknessThe gram-positive bacteria,15-20nm thickness, varies
depends upon the cell. Some of the bacteria contain an 80nm thickness cell wall.
The gram gram-positive bacteria have a 7.5-12nm thickness of the cell wall.
7Muramic Acid
Content
The muramic acid content in the gram-positive bacteria is
16-20%.
The muramic acid content in the gram-negative bacteria is 2-5%.
8ResistantThe cell wall is insoluble in the 1% KOH solution, so it is
resistant to the alkali.
The cell wall is soluble in the 1% KOH solution, so it is sensitive to the alkali.
9Physical
Disruptions
Mostly that the gram-positive cell is highly resistant to the
physical disruptions.
Gram-negative bacteria is less resistant to physicial damage
10S-layer in cell wallIn the cell wall of the gram-positive bacteria, the S-layer is attached to the peptidoglycan layer.n the gram-negative bacteria, the S-layer is attached to the
outer membrane.
11Teichoic acidteichoic acid is present in the cell wall.teichoic acid is absent in their cell wall
12Periplasmic
space
The gram-positive bacteria do not have the periplasmic space.The gram-negative bacteria have a periplasmic space. This differentiates the outer membrane and the plasma
membrane.
13Outer membraneThe cell wall of the gram-positive is single-layered, so it
does not contain the outer membrane.
The cell wall of the gram-
negative is double layered. One is the plasma membrane, and another one is the outer membrane.
14Lipid ContentThe lipid content of the gram-positive bacteria is very low
(1-4%)
The lipid content of the gram-negative bacteria is very high (11-22%).
15Porin channelsThe proteinaceous membrane channels called porins absent
in the gram-positive bacteria.
The proteinaceous membrane channels present in the gram-negative bacteria because it contains the outer membrane.
16LipopolysaccharidesLipopolysaccharides are absent in the layer of the plasma membrane.Lipopolysaccharides are present in the layer
of the outer membrane.
17MesosomesMesosomes are quite prominent in the gram-positives.mesosomes are less
prominent in the gram-negative.
18EndosporesIn the unfavorable stress condition, the gram-positive
bacteria transform into endospores.
The gram-negative bacteria usually do not produce endospores.
19FlagellaThe basal body of the flagella in gram-positive bacteria contains two rings.The basal body of the flagella in gram-negative bacteria contains four rings.
20ToxinsMost of the gram-positive bacteria will produce exotoxins.Most of the gram-negative bacteria produce endotoxins.
21Antibiotic resistanceGram-positive bacteria are
less resistant to antibiotics
Gram-negative bacteria are mostly immune to antibiotics.
22Antibiotic susceptibilitygram-positive cells show high sensitivity to penicillin and sulfonamides antibiotics.gram-negative bacteria
show low susceptible to next-generation penicillin and cephalosporins
antibiotics.
Gram-positives
show low susceptibility to aminoglycoside,
chloramphenicol and tetracycline antibiotics.
The gram-negative bacteria
show high susceptible to
Aminoglycoside, chloramphenicol,
and tetracycline antibiotics.
23Sodium azide resistanceHighly resistant to sodium azide solution.Less resistant to the
sodium azide solution.
24Lysozyme
enzyme
Their cell wall is highly susceptible to degradation by lysozyme enzyme.Their cell wall is less susceptible to the lysozyme enzyme
25Dryness climateGram-positives have
high tolerance to
dryness.
Have less tolerance to dryness.
26Magnetosomesabsent in the gram-
positive bacteria
Some of the gram-negatives have magnetosomes
27PathogenicVery few gram-positive bacteria are pathogenic to
humans and animals.
Most of the gram-negative bacteria are pathogenic to
humans and animals
28ExamplesBacillus substilis,
Streptococcus, Lactobacillus,
Leuconostoc, Clostridium like species
E. coli, Rhizobium, Vibrio, Acetobacter, etc.,
  1. They possess capsules.
  2. Both have the covalently closed circular DNA as the genetic material.
  3. They both contain extra-chromosomal genetic material (plasmids).
  4. Both undergo binary fission as a mode of asexual reproduction.
  5. Both groups included the flagellated (motile) and non-flagellated (non-motile) forms.

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