Archive

Category Archives for "Chemistry"

Chemistry is the science of study of matter in finest sense, its interactions and existence.

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RadioImmunoAssay | The principle and Procedure of RIA

RadioImmunoAssay

Radioimmune assay (RIA): As the name indicates, it is an immunological assay to analyze any antigen or antibody in the patient’s serum to diagnose the disease. This is one of the most sensitive & specific methods of immune assays available. It involves competitive binding of radio-labeled antigen and unlabeled antigen to a high-affinity antibody. The […]

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Qualitative Analysis| 4 Methods & Uses In Chemistry

Qualitative Analysis

Qualitative analysis by definition is the process of estimation or determination of the components present in a given sample. Chemical analysis has two parts as a) qualitative aspect b) quantitative aspect. Qualitative aspect is the primary property determined and quantitative is the next one in most determinations. Qualitative analysis is quality control for many products […]

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Antibiotic sensitivity test procedure

Antibiotic sensitivity test

Antibiotics are natural or semi-synthetic agents which kill bacteria and other microbes at small doses. The number of antibiotics used in present health care system are many yet not all of them are suitable for every kind of infection. For example rifampicin is best for treatment of tuberculous infection while penicillin is best for syphilis. But these two antibiotics are completely ineffective in vice verse manner. This knowledge of selectivity of an antibiotic […]

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What is Electrophoresis | 3 Major Types & Principle in detail

What is Electrophoresis

Electrophoresis is one of the widely used techniques in molecular biochemistry, microbiology, biomedical research. It is a type of protein separation method which relies on protein sizes to segregate the mixture. It is one of the highly effective techniques of analysis and sole method for separation of proteins for western blot, RNA studies, etc. But, […]

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Particle Definition & Importance of Particle Size

Particle Definition

A particle is a fine solid material which is formed either due to condensation of fine solid mass or breakage of large solid mass into smaller single entities. Particle Definition: “Particles are the small and discrete fragments of matter which tend to flow and spread.” They have a set of properties which define the nature of the […]

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Atomic Absorption Spectroscopy | Instrumentation & applications

atomic absorption spectroscopy

Atomic absorption spectroscopy (AAS) is another interesting type of spectroscopy. It is specifically designed for the analysis of the metals and metalloids substances. By definition, AAS is a quantitative analytical technique wherein the absorption of a specific wavelength of radiation by the neutral atoms in the ground state is measured. The more the number of […]

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HPLC method: Procedure of HPLC test in brief

hplc method

HPLC method of chromatography is simple if one follows a well established procedure . Always keep the HPLC instrument clean including the chamber where the HPLC system is housed. Keep the room temperature to lower side and also fixed. Alteration in the room temperature during the HPLC method has effect on the detectors. All the solution, reagents, samples, […]

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Importance of Protein Analysis & the Assay Methods

Importance of Protein Analysis in diagnosis and research

Protein analysis is very important for Quantification of tissue or cell material in biological research. Understand body physiology For diagnosis of diseases by western blot, ELISA. To determine the mechanism of disease Quality control of packed food material and protein supplements. To develop vaccines. Biosynthesis of Hormones and Enzymes Forensic Investigation Identification of bacteria. Protein […]

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