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Category Archives for "Chemistry"

Chemistry is the science of study of matter in finest sense, its interactions and existence.

Gas Chromatography Theory of Operation & Instrumentation

Gas Chromatography Theory

Gas Chromatography Theory:  Gas chromatography relies on the separation of components of a volatile compound. Under the influence of a gaseous solvent flow which acts as mobile phase, the compounds are separated over a liquid stationary phase. The compounds having partition into gas elute first out of the column. While those with greater partition into stationary liquid phase elute out last. The carrier […]

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Gas Chromatography Principle, Instrumentation and Method

Gas Chromatography Principle

Gas chromatography principle is based on “partition” based separation of componenets. This chromatography is of two types like gas liquid chromatography (GLC) and gas solid chromatography (GSC). Of them GLC is widely used and so our entire discussion would be related to it. Gas Chromatography & principle (principle of GC): The principle in gas chromatography involves separation […]

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HPLC Parts; Accessories & their Function

hplc parts

High-performance liquid chromatography is shortly abbreviated as HPLC. This has a sophisticated and complex instrument set up to analyze components. The HPLC parts include an injector, a pump, column and detector. Other accessories include a computer, injector, solvent input and exhaust. Any problems in these parts can ultimately hinder the HPLC process. So it is important to procure […]

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HPLC injection: The technique and instrumentation

HPLC injection

HPLC injection is a technique used to load the sample into the HPLC system. This process is done using a specially designed injection system which is a separate and prominent part of the HPLC system. The most popular injector is the rheodyne injector. The injection is done manually by an external syringe or autoinjector. Then […]

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Hplc Mobile Phase: Requirement and precautions.

In High-performance liquid chromatography (HPLC) method, the mobile phase is of prime importance. It is the component which takes the actual process of separation under the influence of pressure over the stationary phase. The HPLC mobile phase is a bit of concern in comparison to mobile phase of other chromatography techniques due to the following reasons. ♠ […]

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HPLC Applications | In Diagnosis, Research & Industry

HPLC Applications

HPLC (high-performance liquid chromatography) is one of the advanced types of chromatography. It is highly sophisticated and expensive tools in the present analytical chemistry. It is given prominent importance due to its attributes like High sensitivity, i.e., the ability to evaluate samples of very minute concentrations like in nano-gram and picogram Detect precisely chemically similar molecules like […]

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What is Chromatography? Its Definition, Principle & Uses

What is chromatography

What is Chromatography? Chromatography is derived from Greek terms “chromo= color & gram=bands.” Hence as the name indicates, in chromatography, there is the formation of colored bands. These bands are indicative of different components in the sample. In the Initial stages, it was developed as column chromatography to separate mixtures. Here into a long vertical column, sample along with a mobile phase was allowed […]

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What is HPLC GRADE & its importance in HPLC ?

High pressure liquid chromatography is one of the widely used analytical technique. But it very sensitive to operate at constant pressure. Further the mobile phase passes through a dense column so all the material used in the procedure should be of high quality. While performing analysis by HPLC chromatography, one is advised to use HPLC […]

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Chromatography Lab: Minimal and essential requirements

Chromatography lab

Chromatography labs vary largely based on the type of  chromatography method used. They are designed in a way suitable for the particular chromatographic procedure. But all the chromatography labs have some common requirements to ensure the sensitivity, reproducibility and reliability in analytical results. Since chromatographic procedure is one of the mandatory requirement in some researches, it is necessary to provide a […]

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